ACTIVE INGREDIENTS

ACTIVE INGREDIENTS
Use in Biogreen Science Product

1. Phytocelltec™ MALUS DOMESTICA

PhytoCellTec™ Malus Domestica is a liposomal active ingredient based on stem cells from the Uttwiler Spatlauber apple.

apple stem cell

The Apple
The Uttwiler Spatlauber is an old Swiss variety, which is very rare today. It was known for its exceptional capacity to keep for long periods but gradually lost its popularity to sweet apples because of its bitter taste. As the apple could be stored for a long time without becoming shriveled or losing flavor, it clearly had special ingredients and especially long-lived stem cells. And precisely these longevity factors were of particular interest to the researchers at Mibelle Biochemistry.

Plant stem cells for skin stem cells
The development of the innovative PhytoCellTec™ technology made possible to grow plant stem cells on a large scale. It also became possible to effectively cultivate rare and endangered plants like the Uttwiler Spatlauber. PhytoCellTec™ made plant stem cells available for cosmetics for the first time.

The effect
Mibelle Biochemistry was able to show in comprehensive studies that PhytoCellTec™ Malus Domestica, the active ingredient based on apple stem cells, promotes the vitality and longevity of skin stem cells, protects them from damage due to environmental stress and thus delays the skin aging processes.

Summary
PhytoCellTec™ Malus Domestica has opened up entirely new possibilities in the field of cosmetics. Using plant stem cells can slow skin aging by protecting essential skin stem cells, keeping the skin looking youthful longer, and giving it a better and more vital appearance.

Improves the vitality of stem cells
Cultures of epidermal stem cells were treated with different concentrations of PhytoCellTec™ Malus Domestica and their colony-forming efficiency was determined. The investigations showed that PhytoCellTec™ Malus Domestica had a clear, positive effect on stem cell characteristics.

Preserves the ability to form new epidermal tissue
Young, vital epidermal stem cells can form a complete, multi-layer epidermis in laboratory conditions. When this experiment is conducted on older stem cells, these no longer multiply and no epidermis is formed. These stem cells are depleted and have reached the end of their lifecycle. If, however, Malus Domestica stem cell extract is added to cultivate the stem cells, even “old” stem cells can still generate a stratified epidermis. Under the influence of Malus Domestica stem cell extract, the epidermal stem cells retain their vitality longer and are capable of forming new tissue and renewing the skin.
(see chart)

Anti-wrinkle effect
The anti-wrinkle effect of PhytoCellTec™ Malus Domestica was studied in a clinical trial with 20 subjects (aged 37 to 64). An emulsion with 2% PhytoCellTec™ Malus Domestica was applied to the crow’s feet area over 28 days. The results showed a significant, visible reduction in wrinkles in 100% of the subjects. (see figure)
Source Mibelle Biochemistry

anti-wrinkle

 

 

2. Phytocelltec™ SOLAR VITIS/VITIS VINIFERA

PhytoCellTec™ Solar Vitis is based on stem cells from the Gamay Teinturier Freaux grape – a grape from Burgundy, which is characterised by an extremely high content of polyphenols for UV protection.

solarvitis-grape
Extensive studies have shown that PhytoCellTec™ Solar Vitis reliably protects epidermal stem cells from UV stress and thus from skin aging caused by light. PhytoCellTec™ Solar Vitis increases the vitality and efficiency of all essential skin cells and improves the skin’s resistance.

Summary
The use of plant stem cells protects the most important skin cells, namely its stem cells, thus delaying skin aging. PhytoCellTec™ Solar Vitis protects the skin from chronological and light-induced aging, extending skin cells’ vitality and keeping the skin’s appearance young and beautiful longer.

Improves the vitality of stem cells
Cultures of epidermal stem cells were treated with different concentrations of PhytoCellTec™ Solar Vitis and their colony-forming efficiency was measured. The research demonstrated that PhytoCellTec™ Solar Vitis clearly has a positive effect on stem cell characteristics.
(see chart)
Protects epidermal stem cells from UV stress
Cultures of epidermal stem cells were treated with PhytoCellTec™ Solar Vitis, while others were left untreated. The cultures were exposed to UVA and UVB rays, and the colony-forming efficiency (CFE) of the stem cells was then measured. The results showed that UV radiation strongly reduced cells’ colony-forming efficiency (-58%). Treatment with PhytoCellTec™ Solar Vitis improved the colony-forming ability of the stem cells, and the cells’ vitality was not impaired even after exposure to UV radiation. (see chart)

solar-protect

3. Phytocelltec™ ALP ROSE
PhytoCellTec™ Alp Rose is a cosmetic ingredient based on stem cells of alpine rose leaves.

rose
The Alpine rose
The alpine rose is a typical Swiss flowering plant from the Alps. It has bright pink blossoms and grows at altitudes up to 3200 m. Its evergreen leaves are especially resistant to high UV radiation and extreme dryness and cold. The sturdy leaves are a shiny green, even once the snow has melted in the spring. This phenomenon is due to the presence of a special protection protein called dehydrin in the leaves of the alpine rose.

Plant stem cells for skin stem cells
For years researchers at Mibelle Biochemistry have investigated the compounds of the alpine rose and its special protective substances, in particular, the water-retaining dehydrin. The innovative PhytoCellTec™ technology has been used to develop a formula from the leaves’ stem cells that treats dry and stressed skin and has been proven to protect skin cells: PhytoCellTec™ Alp Rose.

The effect
Mibelle Biochemistry was able to show in extensive studies that PhytoCellTec™ Alp Rose promotes the vitality and longevity of skin stem cells. It strengthens the skin barrier, providing better protection to the skin against climate-induced stress.

Summary
The effect of PhytoCellTec™ Alp Rose combines two important approaches. Using plant stem cells delays skin aging by protecting vital skin stem cells, keeping the skin looking youthful longer, and giving it a better and more vital appearance. At the same time, PhytoCellTec™ Alp Rose protects the skin using special protective ingredients from this extremely resistant alpine plant.

 Protects epidermal stem cells from UV stress

Cultures of epidermal stem cells were treated with PhytoCellTec™ Alp Rose, while others were left untreated. The cultures were then exposed to UVA and UVB rays. The colony-forming efficiency (CFE) of the stem cells was then measured. The results showed that UV radiation strongly reduces the cells’ colony-forming efficiency. Treatment with PhytoCellTec™ Alp Rose increased the stem cells’ colony-forming efficiency (vitality) and provided significant protection against UV rays.

 Ski holiday study

Twenty-two subjects were treated with an SPF 30 sunscreen formula during their ski holidays (1 week in March, Alps) to test the effect of PhytoCellTec™ Alp Rose in extreme environmental conditions. Sunscreen without the active ingredient was applied to one side of a subject’s face, while the other side was treated with sunscreen containing the active ingredient PhytoCellTec™ Alp Rose. The loss of skin moisture in the face was then measured after another week of treatment at home. The results showed that water loss was reduced by 39% after 2 weeks’ treatment with PhytoCellTec™ Alp Rose. This indicates that PhytoCellTec™ Alp Rose strengthens the skin barrier and makes it more resistant to environmental stresses such as UV, wind, cold temperatures and dryness. (see chart)

rose-holiday

 

 

4. Phytocelltec™ ARGAN

PhytoCellTec™ Argan is based on stem cells of the Argan tree, one of the oldest tree species in the world.

Argan-tree
Phytocelltec™ Argan
PhytoCellTec™ Argan was developed to improve the regenerative capacity of dermal stem cells thereby achieving deep-seated rejuvenation of the skin. This active ingredient is based on stem cells of the Argan tree, one of the oldest tree species in the world. The argan tree is indigenous to the arid southwest of Morocco and has adapted perfectly to intense drought and extremely high temperatures, which are typical in this region. Unfortunately, the Argan tree population has shrunk dramatically due to extensive cultivation of the land, so that nowadays the remaining Argan forests are under the protection of UNESCO.
Adult skin stem cells – Dermal stem cells
The main function of the dermis is to provide mechanical support and elasticity to the skin. In order to maintain these important functions, healthy skin undergoes constant cell turnover, which renews and repairs the dermal connective tissue. New cells are produced to replace damaged or dead ones. Responsible for this regenerative process are adult dermal stem cells, which reside in the dermal papilla of the hair follicle. These cells differentiate into dermal cell types like fibroblasts, which are responsible for the continuous production of the extracellular matrix proteins elastin and collagen. However, dermal stem cells do age, they become less in number and exhibit reduced activity. As a consequence the production of collagen and elastin drops and the skin loses its firmness – wrinkles appear.
Therefore it is important to protect the dermal stem cells and to maintain their activity.

A new assay to measure dermal stem cell activity
In order to evaluate the activity of PhytoCellTec™ Argan on dermal stem cells, a stable human dermal stem cell line was used as a new test system: stem cell activity is assessed based on the expression of the stem cell marker Sox2 (a key regulator of pluripotency in dermal stem cells) and on the property of dermal stem cells to grow in three-dimensional spherical colonies. These two characteristics are only expressed by vital dermal stem cells.
Dermal stem cells, which are cultivated in the presence of the Argan stem cell extract show enhanced Sox2 expression compared to the untreated control culture (figure 1). Moreover, when seeded on a culture dish these “aged” stem cells exhibit a better ability to form 3-D spheres than the untreated control (figure 2). Thus, PhytoCellTec™ Argan helps the dermal stem cells to maintain their “stemness” even after long-term cultivation.

figure1-arganfigure2-argan
Improvement of the extracellular matrix structure – reduction of wrinkle depth
The dermal tissue structure of forearms from volunteers was visualized by ultrasonography before and after treatment with PhytoCellTec™ Argan (figure 3). In the ultrasonographic images the dermis appears in bright colours, whereas the so-called subepidermal low echogenic band (SLEB) produces dark patches. This SLEB is the result of a disrupted architecture of the dermis and commonly found in aged and photo-damaged skin. Results clearly show, that a 56 days treatment with PhytoCellTec™ Argan reduces the SLEB and significantly improves the dermal tissue density.
Figure 4 shows ultrasonographic images made from a women’s thigh, where the dermis is depicted in bright colours and the hypodermis shows up in black. The irregular dermis-hypodermis junction is caused by adipose tissue protruding into the lower dermis (figure 3, red arrows). This is a typical image for cellulite. A 56 days treatment with PhytoCellTec™ Argan shows a tightening of the dermal tissue, leading to a much smoother dermis-hypodermis junction.
The anti-wrinkle effect of PhytoCellTec™ Argan was demonstrated in a clinical study with 21 volunteers, average age 49. A cream with 0.4% PhytoCellTec™ Argan was applied to the crow’s feet area around the eye over 56 days. The results reveal a reduction in wrinkle depth by remarkable 26 %.
PhytoCellTec™ Argan is the first active ingredient capable of both protecting and vitalizing human dermal stem cells. This not only helps to accelerate the skin’s natural repair process but also fights skin aging right at the root for a wonderfully fresh and radiant appearance.

figure3-arganfigure4-argan

 

source :Mibelle Biochemistry 

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